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Expressions of ARSI negatively correlated with tumor mutation burden or microsatellite instability and positively correlated with immune-related genes. Elevated ARSI expressions conferred poor tolerance to daporinad and sinularin, but increased cell sensitivity to dasatinib and XAV939. Arylsulfatase I is a promising prognostic and therapeutic target for HNSC. Arylsulfatase I is a promising prognostic and therapeutic target for HNSC. To establish a highly sensitive time-resolved fluorescence immunoassay (TRFIA) of kidney injury molecule