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Calcium level of the SO2 group; OPG and calcium of O3 group; and PTH of AAP group were less than those of control group but differences were not significant (p0.05). The results showed significant effect of AAP with natural daylight on vitamin D and also O3 on PTH of the rats. In the concentrations and conditions of the study, we didn't find any significant unwanted effects of AAP, SO2, and O3 on the bone biochemical parameters. More investigations with more concentrations and exposure time are recommended.This study investigated t