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04). The relative abundance of microbiota was not different among patients with achalasia from those with ESCC. The oral and esophageal microbiota were significantly different in patients with achalasia, and some of the composition of the esophageal microbiota changed after POEM. However, these findings and disease-specific microbiota should be further evaluated in large-scale studies. The oral and esophageal microbiota were significantly different in patients with achalasia, and some of the composition of the esophageal microbiota chan