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The aim of our study is to estimate the abundance and sources of floating microplastics (MPs) along a coastal lagoon ecocline in a marine conservation unit (MCU) for implementing effective prevention and mitigation actions in South Brazil. MPs were sampled monthly, and the abundance and size were determined for hard solids, soft plastic, plastic filaments, and paint fragments. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed, and the MPs were classified as polyethylene, polyester, po