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We report the design of a setup combining the simultaneous and independent optical trapping and second-harmonic generation (SHG) of 1 µm diameter silica microspheres with two independent laser beams. Optical trapping is achieved with a tightly focused continuous wave infrared laser beam whereas the SHG intensity from the trapped microparticles is obtained with a 810 nm femtosecond pulsed laser. The silica microparticles are dispersed in an aqueous solution, and a microfluidic channel flow is used to remove untrapped microparticles. We
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