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Enzyme-linked immunosorbent assay (ELISA) with flat solid substrates is dominantly found in finding whole viruses because of its straightforwardness and user friendliness in assay protocols, however it usually is affected with minimal sensitiveness, poor measurement range, and a time-consuming assay treatment. Here, a lipid-nanopillar-array-based immunosorbent assay (LNAIA) is developed with a nanopillar-supported lipid bilayer substrate with fluorophore-modified antibodies for qui