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Hypertonic saline induced moderate thigh pain at rest (NRS 4.6±2.1) compared to the control injection (NRS 0.3±0.4; p0.001). EIH at the thighs and neck/shoulder were not different between sessions (Injected thigh 0kPa; 95% CI-51 to 52; Contralateral thigh-6kPa; 95% CI-42 to 30; neck/shoulder 19kPa; 95% CI-6 to 44). No significant associations between pain intensity ratings immediately after the Painful injection and EIH responses at any assessment sites were found (right thigh =0.08, 95% CI-12.95 to 20.64, p=0.64, left thigh =-0.33,