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1 m3/m2-d, respectively. On the other hand, substantial enhancement in the nitrate nitrogen (NO3 --N) in the effluent (73 ± 10.55 times its influent concentration) was observed with influent COD of 200 ± 25 mg/L and HLR of 7 ± 0.2 m3/m2-d. When the influent COD and HLR were maintained at 700 ± 45 mg/L and 3 ± 0.1 m3/m2-d, respectively, the highest total nitrogen removal of 87 ± 2.25% was obtained. Alternatively, the influent COD of 200 ± 25 mg/L and HLR of 3 ± 0.1 m3/m2-d yielded the highest COD removal efficiency of 77 ± 1.59%. Hence,