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A nucleocapsid protein (NP)-based enzyme-linked immunosorbent assay (ELISA) detection method is not only essential in illness diagnosis, it is needed for the analysis of vaccine effectiveness throughout the development of an inactivated SARS-CoV-2 vaccine. In this study, we indicated both the NP and N-terminally truncated NP (ΔN-NP) of SARS-CoV-2 in an Escherichia coli phrase system and described the purification of the dissolvable recombinant NP and ΔN-NP in details. The identiti