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The aim of present study was to assess the cytotoxicity and antimicrobial efficacy of 2% peracetic acid (PAA) compared with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX). For the cytotoxicity test, 100 µl of the tested solutions were added in 12 wells with ECV 304 endothelial cells in each group NaOCl, CHX and PAA, in addition to the control group. Each solution was evaluated after 24 hours of contact in four dilutions 0.2, 0.1, 0.05 and 0.025 through mitochondrial function using MTT colorimetric assay. In