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The work synthesized the fungal chitosan (FCS) encapsulated Gynura procumbens (GP) mediated silver nanoparticles (GP-AgNPs) for enhanced antidiabetic, antioxidant and antibacterial activity. The FCS-GP-AgNPs were characterized through UV-Visible spectroscopy, FTIR, XRD, Zeta size analyzer and TEM. The FTIR spectrum of GP-AgNPs exhibited functional groups of phenolic and flavonoids. The crystal peaks related to silver and chitosan in FCS-GP-AgNPs were demonstrated by XRD spectrum. The polydispersed nanoparticles such as AgNPs and FCS-GP-Ag