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Functionally, experimental airway allergy augmented the excitatory airway vagal response to intracisternally injected GABA, that has been attenuated by intracisternally pre-injected NKCC1 inhibitor bumetanide. All of the changes caused by experimental airway allergy had been prevented or mitigated by chronic intracerebroventricular or intraperitoneal injection of minocycline, an inhibitor of microglia activation. These outcomes demonstrate that experimental airway sensitivity augme