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glabrata, 14 cases of C. albicans, one case of C. tropicalis, and 0 cases of C. krusei. The frequency of S. aureus, P. aeruginosa, and Candida spp. increased after the orthodontic treatment in either the ROA and or in the support oral mucosa. There is a direct relation between the use of the ROA and the increase of periodontal-pathogenic microorganisms. The frequency of S. aureus, P. aeruginosa, and Candida spp. increased after the orthodontic treatment in either the ROA and or in the support oral mucosa. There is a direct relation betw