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The MB-labelled particles emitted a stronger fluorescent sign at 662 nm. We found that the fluorescent light emission and steric stabilization made this PBNP-MB particle platform relevant for in vivo optical imaging. (4) Conclusion. We effectively produced a fluorescent and steady, Prussian blue-based nanosystem. The particles may be used as a platform for imaging contrast improvement. In vivo stability and biodistribution studies revealed brand-new aspects of making use of