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Its effect of inducing apoptosis mediated via concentrating on PTEN/PI3K/AKT signalling path were additionally analyzed along with cell migration and intrusion. Practices Cell proliferation had been tested by MTT mobile viability assay. Fluorescence microscopy had been employed to perform apoptosis associated studies via DAPI staining along with movement cytometry using annexin V/propidium iodide (PI) assay. Further, western blotting evaluation had been carried out to look
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